Research Paper Volume 12, Issue 21 pp 22046—22058

Figure 3. miR-205 restored the effect of THBS1 overexpression on cells. (A, B) The viability of the indicated cells was detected by MTT assay and colony formation assay. **P < 0.05, vs. scramble+vector. ##P < 0.05, vs. scramble+THBS1. ^^P<0.05, vs. miR-205+vector (C, D) The apoptosis of the indicated cells was detected by Hoechst 33258 and Annexin V-PI assay. **P < 0.05, vs. scramble+vector. ##P < 0.05, vs. scramble+THBS1. ^^P<0.05, vs. miR-205+vector (E) The indicated cell migration was measured by Transwell assay. **P < 0.05, vs. scramble+vector. ##P < 0.05, vs. scramble+THBS1. ^^P<0.05, vs. miR-205+vector (F, G) Expression of the indicated genes was measured by Western blot and real-time PCR, respectively. The cells were transfected with scramble plus vector, scramble plus THBS1, miR-205 plus vector, and miR-205 plus THBS1. Results show the mean ± SD of three independent experiments.