Research Paper Volume 12, Issue 22 pp 23096—23113

Figure 4. DLX6-AS1 was upregulated in an OGD/R model and DLX6-AS1 silencing reduced apoptosis of N2a and SH-SY5Y cells induced by OGD/R. (A) Representative images and statistics of TUNEL staining of N2a cells used to confirm apoptotic changes (100X). (B) Cleaved caspase-3 protein levels measured by western blotting. (C) Representative images and statistics of TUNEL staining of SH-SY5Y cells used to confirm apoptotic changes (100X). (D) Cleaved caspase-3 protein levels of SH-SY5Y measured by western blotting. Values represent mean ± SEM (n = 3 in each group). **P < 0.01, ***P < 0.001 vs Ctrl; +P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 vs OGD 3 h/R 24 h + NEG.