Research Paper Volume 12, Issue 19 pp 19809—19827
Thioredoxin protects mitochondrial structure, function and biogenesis in myocardial ischemia-reperfusion via redox-dependent activation of AKT-CREB- PGC1α pathway in aged mice
High level of Trx protects against I/R- induced apoptosis. (A) Cytosolic extract was prepared from AAR region of sham or I/R subjected NT, Trx-Tg and dnTrx-Tg mice and level of Cyt-C was analyzed by western blotting. (B) Levels of cytosolic Cyt-C was quantified and expressed as fold change. *p <0.05 versus NT sham; **p <0.05 versus NT or dnTrx-Tg I/R; † p <0.05 versus NT or Trx-Tg I/R. (C) AAR region of sham or I/R myocardium from NT, Trx-Tg and dnTrx-Tg were lysed using M-PER lysis buffer and analyzed for cleaved caspase 3 and Bax by western blotting. (D) Protein levels were quantified and expressed as fold change. *p <0.05 versus NT sham; **p <0.05 versus NT or dnTrx-Tg I/R hearts, n=3. (E) EPR spectra of paramagnetic iron bound Annexin-V. (F) Graph shows an absolute spin count of Fe-Annexin-V. Values are means ± SD (n = 3 mice). *, p < 0.01 versus NT Sham; **, p < 0.01 versus NT I/R or dnTrx-Tg I/R; † p <0.01 versus NT or Trx-Tg I/R. Statistical significance was determined with the Student’s t test (B, D) and one-way ANOVA followed by Tukey’s post-hoc multiple comparisons test (F).