Research Paper Volume 13, Issue 2 pp 1947—1961

Figure 4. EPB41L3 knockdown promotes migration and invasion of osteosarcoma cells. (A) Metastasis-free survival analysis of patients from GSE42352 (p=0.0450) in the R2 online database (http://r2.amc.nl). (B) EPB41L3 mRNA expression was significantly higher in primary osteosarcoma cells than lung metastasis cells from GSE85537. (C) Wound-healing assays for U2OS and MG63 cells stably expressing shNC or shEPB41L3. (D) U2OS and MG63 cells stably expressing shNC or shEPB41L3 were subjected to Boyden’s chamber migration assays. Representative images of cell migration were presented (magnification, x100) and migratory cells were counted. (E) U2OS and MG63 cells stably expressing shNC or shEPB41L3 were subjected to Matrigel-coated invasion assays. Representative images of cell invasion were presented (magnification, x100) and invasive cells were counted. All experiments were repeated three times. Data were presented as the mean ± SD.