Research Paper Volume 13, Issue 4 pp 4999—5019

Figure 2. HOXB9 promotes migration and invasion in NSCLC cells. (A, B) HOXB9 knockdown was performed in H1915 and H1299 NSCLC cells by transfection of HOXB9-siRNA. Scrambled siRNA was used as negative control. HOXB9 knockdown efficiency was determined using western blot, gray level analysis and qPCR. (C, D) Cell proliferation was determined by the CCK-8 assay in H1915 and H1299 cells at 24, 48, and 72 h after transfection with HOXB9-siRNA or scrambled siRNA. HOXB9 knockdown did not affect proliferation in any cell line. (E, F) Representative images from wound-healing migration assays in H1915 and H1299 cells. (G, H) Quantification of wound-healing assay results shows decreased migratory potential after siRNA-mediated HOXB9 knockdown (p = 0.0036 and p = 0.0079 for H1915 and H1299 cells, respectively). (I) Representative images from Transwell invasion assays in H1915 cells and H1299 cells. (J, K) Quantification of Transwell invasion assay data showing that HOXB9 knockdown significantly inhibited invasive potential in both cell lines (p = 0.0037 and p = 0.0005 for H1915 and H1299 cells, respectively).