Leaky severe combined immunodeficiency in mice lacking non-homologous end joining factors XLF and MRI

Sergio Casta&#xF1;eda-Zegarra; Qindong Zhang; Amin Alirezaylavasani; Marion Fernandez-Berrocal; Rouan Yao; Valentyn Oksenych

doi:doi:10.18632/aging.202346

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Research Paper Volume 12, Issue 23 pp 23578—23597

Leaky severe combined immunodeficiency in mice lacking non-homologous end joining factors XLF and MRI

Figure 2. Development of B cells is abrogated in bone marrow of Xlf^-/-Mri^-/-Trp53^+/- and Xlf^-/-Paxx^-/-Trp53^+/- mice. (A) Flow cytometric analysis of developing B cells. Upper left boxes mark B220+CD43+IgM- progenitor B cell populations, and lower right boxes mark the B220+CD43-IgM+ B cells. (B, C) Frequencies (%) of B220+CD43-IgM+ B cells (B) and B220+CD43+IgM- progenitor B cells (C) in WT, Xlf^-/-, Mri^-/-, Xlf^-/-Mri^-/-Trp53^+/-, Paxx^-/-, Xlf^-/-Paxx^-/-Trp53^+(-)/- and Paxx^-/-Mri^-/- mice. Comparisons between groups were made using one-way ANOVA, GraphPad Prism 8.0.1. Xlf^-/-Paxx^-/-Trp53^+(-)/- is a combination of Xlf^-/-Paxx^-/-Trp53^+/- and Xlf^-/-Paxx^-/-Trp53^-/-.