Research Paper Volume 13, Issue 4 pp 5967—5985

Figure 1. The hUCMSCs and their derived hUCMSC-ex are successfully isolated. (A) Identification of hUCMSCs after 1 week of culture and at passage 3. (B) Expression of mesenchymal cell surface markers (CD29, CD44, HLA-I, CD34, CD38 and HLA-DR) detected by flow cytometry. (C) Immunofluorescence assay detected the neurogenic induction of hUCMSCs. (D) Oil red O staining measured the adipogenic induction of hUCMSCs. (E) Alizarin red staining measured the osteogenic induction of hUCMSCs. (F) TEM observation of morphology of exosomes. (G) Particle size of exosomes calculated using ZETASIZER Nano series-Nano-ZS. (H) Protein expression of CD63 and CD81 in isolated hUCMSC-ex measured by Western blot assay. All the experiments were repeated 3 times. Data in panel (H) were analyzed by two-way ANOVA and the pairwise comparisons after ANOVA were performed with Tukey's multiple comparisons test. *p < 0.05.