Research Paper Volume 13, Issue 4 pp 6041—6054

Figure 3. CERS6-AS1 sequesters miR-15a-5p in PDAC cells. (A) The location of CERS6-AS1 predicted by lncLocator. (B) CERS6-AS1 distribution in the cytoplasmic and nuclear fractions of PANC-1 and SW1990 cells. (C) The potential miRNAs of CERS6-AS1 predicted by StarBase 2.0. (D) Relative miRNA expression was detected in PANC-1 and SW1990 cells after CERS6-AS1 knockdown. (E) miR-15a-5p expression in paired PDAC tissues and adjacent non-tumor tissues was detected by RT-qPCR. (F) The correlation between miR-15a-5p and CERS6-AS1 expression in PDAC tissues was examined by Pearson’s correlation coefficient analysis. (G) The wild-type and mutant miR-15a-5p binding sites in CERS6-AS1. (H) PANC-1 and SW1990 cells were transfected with CERS6-AS1-wt or CERS6-AS1-mut reporter vectors and miR-15a-5p mimic or NC mimic, and a luciferase reporter assay was conducted to measure the luciferase activity. (I) PANC-1 and SW1990 cells were incubated with Ago2 or IgG, and then immunoprecipitated RNA was analyzed by RT-qPCR to evaluate miR-15a-5p and CERS6-AS1 enrichment. **P < 0.01.