Research Paper Volume 13, Issue 7 pp 9627—9645

Hsa_circ_0053063 inhibits breast cancer cell proliferation via hsa_circ_0053063/hsa-miR-330-3p/PDCD4 axis


Figure 6. PDCD4 served as a molecular target of miR-330-3p. (A) Putative complementary sites within PDCD4 and R-330-3p predicted by TargetScan. (B) Dual-luciferase reporter assay evaluates the interaction between wild-type PDCD4 + miR-330-3p reporters compared with mutant PDCD4+ miR-330-3p reporter. (C–D) The expression levels of PDCD4 were tested in both BC tissues and cell lines through QRT-PCR. (E–H) Effect of siRNA targeting PDCD4 on colony formation and MTT assays in MDA-MB-231 and MDA-MB-468 cell lines. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).