Research Paper Volume 13, Issue 7 pp 9748—9765
Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p
Figure 5. Upregulation of DPP10-AS1 and downregulation of miR-127-3p suppress the proliferation and stimulate apoptosis of CCSCs. (A, B) cell proliferation detected by CCK-8 assay after DPP10-AS1 expression was altered; (C, D) cell apoptosis detected by flow cytometry after DPP10-AS1 expression was altered; (E, F) cell proliferation detected by CCK-8 assay after miR-127-3p expression was altered; (G, H) cell apoptosis measured by flow cytometry after miR-127-3p expression was altered. *, p < 0.05 vs. vector and si-NC, or NC mimic and NC inhibitor. The measurement data were expressed as mean ± standard deviation. The data among multiple groups were analyzed by two-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times. DPP10-AS1, cells transfected with DPP10-AS1 plasmid; si-DPP10-AS1, cells transfected with si-DPP10-AS1; si-NC, cells transfected with si-negative control plasmid; miR-127-3p mimic, cells transfected with miR-127-3p mimic plasmid; NC mimic, cells transfected with negative control mimic plasmid.