Research Paper Volume 13, Issue 7 pp 9991—10014

Transcriptome and lipidome profile of human mesenchymal stem cells with reduced senescence and increased trilineage differentiation ability upon drug treatment


Figure 2. Senescence alleviation and trilineage differentiation improvement of drug inducted BM-hMSCs. (A) Morphology of BM-hMSCs in day 10 after drug induction. Scale bar, 100 μm. (C) SA-β-gal staining of BM-hMSCs inducted with the indicated drugs. (E) Oil red O staining on day 25 after transduction to label adipocyte. Scale bar, 200 μm. (B), (D), and (F) ImageJ was used for image analysis. Pictures were quantified by measuring the positive staining area. Data are presented as the mean ± SEM, n > 3, *P < 0.05, **P < 0.01, ***P < 0.001.