Research Paper Volume 13, Issue 7 pp 10275—10288

CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis–angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF


Figure 1. CircRNA hsa_circ_0006215 downregulates in BMSCs isolated from patients with osteoporosis. (A) Heat map of circRNA RNA-seq. Expression of circRNAs using qRT-PCR in (B) BMSCs from patients with and without senile osteoporosis and (C) BMSCs during osteogenic differentiation. Expression of hsa_circ_0006215 detected by qRT-PCR (D) on days 0, 1, 3, 7 and 14 of osteogenic differentiation and (E) in nucleus and cytoplasm. (F) Distribution of hsa_circ_0006215 in BMSCs detected by FISH. (G) Expression of hsa_circ_0006215 in BMSCs at different passages detected by qRT-PCR. Results are expressed as means ± SD of three independent experiments. *p < 0.05, **p < 0.01. FISH, fluorescent in situ hybridization.