Research Paper Volume 13, Issue 7 pp 10312—10325

Figure 1. Membrane-cytoplasm translocation of ANXA4 in CRCA. (A) ANXA4 mRNA expression levels were significantly higher in colorectal cancer tissue samples than in normal tissue samples, as indicated by analysis of the Oncomine colorectal cancer dataset, Skrzypczak Colorectal dataset and Sabates-Bellver Colon dataset. (B) Representative images of the protein expression of ANXA4 in CRC and tumour-adjacent tissue samples (n=138) generated by immunohistochemistry. (C) Representative blots of the membrane and cytoplasmic protein expression of ANXA4 in CRC and tumour-adjacent tissue samples (n=19) generated by western blotting. Tubulin was used as the loading control for total protein; Na+/K+-ATPase was used as the loading control for cell membrane protein. (D) The expression of ANXA4 in normal colon cells (NCM460) and colorectal cancer cells (SW480, SW620, and HT-29 cells) determined by western blotting. Tubulin was used as the loading control for total protein. (E) The membrane and cytoplasmic protein expression levels of ANXA4 in HT-29 cells determined by western blotting. Na/K-ATPase was used as the loading control for cell membrane protein; Tubulin was used as the loading control for total protein. (F) Correlation analysis between high ANXA4 expression and metastasis in CRC patients (n=138) by chi-square test analysis (p<0.05).