Research Paper Volume 13, Issue 7 pp 10703—10723

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration


Figure 4. The effect of acidic culture conditions on the proliferation of human NP-MSCs. The proliferation ability of human NP-MSCs derived from patients with Pfirrmann grade II (A), III (B) and IV (C) degeneration was determined using CCK-8 assays under control conditions or after exposure to extracellular acid (pH6.6) alone and in combination with addition of the inhibitors Amiloride, PcTx1 or APETx2. n=3 for each group; data are mean ± SD.