Research Paper Volume 13, Issue 8 pp 11727—11737

Figure 4. MiR-27a-3p directly targets FOXO1 in HT22 cells. (A) Gene structure of FOXO1 at position 426-432 indicates the predicted target binding site of miR-27a-3p in its 3'UTR. (B) Luciferase activity measured after co-transfecting with wild-type or mutant FOXO1 3′-UTR plasmid and miR-27a-3p mimics into HT22 cells. The results were normalized to Renilla luciferase activity. (C) RT-qPCR analysis of FOXO1 expression in HT22 cells. ^**P<0.01 vs. control. ^##P<0.01 vs. OGD.