Research Paper Volume 13, Issue 6 pp 8040—8054

Figure 1. Heat-inactivated Lb. fermentum BGHV110 affects longevity and healthspan in C. elegans. (A) Lifespan curve of WT animals fed with heat-inactivated control (OP50) and BGHV110 bacteria from the L4 developmental stage maintained at 20° C (n=100 per group, results from one of two experiments with similar results are shown). (B) Body bending rates were measured in WT animals on day 5 and day 10 of adulthood (n=10 per group, results are representative of 3 independent assays). (C) Gut localization and (D) fluorescence intensity quantification of acridine orange-stained heat-inactivated OP50 and BGHV110 bacteria visualized by fluorescence microscopy in day 1 adult WT animals (n=10 per group, results are representative of 3 independent assays). (E) Pharyngeal pumping rates were measured in WT animals on day 5 and day 10 of adulthood (n=10 per group, results are representative of 3 independent assays). All values are presented as mean ± SD. Student’s t-test was used to compare the treated group relative to control (*p < 0.05). The log-rank (Mantel-Cox) test was used to assess the p-value in lifespan analysis.