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Research Paper Volume 13, Issue 8 pp 12031—12045

Low magnitude vibration alleviates age-related bone loss by inhibiting cell senescence of osteogenic cells in naturally senescent rats

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Figure 2. Senescence identification of BMSCs in aged and young rats. (A) 53BP1 immunofluorescence staining of aged and young rat BMSCs; (B) H3K9Me3 immunofluorescence staining of aged and young rat BMSCs; (C) Senescence marker protein assay and relative protein expression statistics of aged and young rat BMSCs (x¯±s,n=3); (D) β-Galactosidase staining plots and statistical plots of the percentage of positive β-galactosidase staining cells (x¯±s,n=3); *P < 0.05 vs. young female rats, **P < 0.01 vs. young female rats, #P < 0.05 vs. young male rats, ##P < 0.01 vs. young male rats.