Research Paper Volume 13, Issue 8 pp 12031—12045

Figure 4. Senescence identification of osteocytes in aged and young rats. (A) 53BP1 immunofluorescence staining of aged rat and young rat osteocytes; (B) H3K9Me3 immunofluorescence staining of aged rat and young rat osteocytes; (C) Senescence marker protein assay and protein relative expression statistics of aged rat and young rat osteocytes (x¯±s,n=3); (D) β-Galactosidase staining of aged rat and young rat osteocytes and statistical plots of the percentage of positive β-galactosidase staining cells (x¯±s,n=3); *P < 0.05 vs. young female rats, **P < 0.01 vs. young female rats, ^#P < 0.05 vs. young male rats, ^##P < 0.01 vs. young male rats.