Research Paper Volume 13, Issue 9 pp 12479—12492

Figure 2. SUMO1P3 upregulation endowed the malignancies of normal liver cells. LO2 cells were not transfected (control) or transfected with pcDNA3.1-SUMO1P3 or pcDNA3.1 plasmids. (A) The expression of SUMO1P3 was detected by qPCR assays. GAPDH was used as the endogenous control. (B) EdU staining was used to detect cell proliferation. Scale bar: 5 μm. (C) Percentage of EdU-positive staining in (B). Transwell assays were performed to evaluate the migration (D) and invasion (F) of MHCC97H and HepG2 cells. 100 × magnification in (F). The numbers of migrated (E) and invaded (G) cells were calculated. (H) LO2 cells subjected to 24 h of serum starvation were untransfected (control) or transfected with pcDNA3.1-SUMO1P3 or pcDNA3.1 plasmids. Caspase-3 activity was detected by using a commercial kit. All data are represented as the mean ± SD of three replicates. *P < 0.05, **P < 0.01 vs. Ctrl or pcDNA3.1 group.