Research Paper Volume 13, Issue 9 pp 12479—12492

Figure 4. SUMO1P3 silencing induced HCC cell apoptosis in vitro. MHCC97H and HepG2 cells were not transfected (control) or transfected with siSUMO1P3-2 or siNC. Cell apoptosis was measured via TUNEL (A, B), DNA fragmentation (C) and flow cytometry (D) assays. Scale bar in (A): 10 μm. (E) The percentage of apoptotic cells in (D) was assessed. (F) Caspase-3 activity was detected by using a commercial kit. (G) The levels of Bax, Bcl-2, cl-caspase-3, and caspase-3 were analyzed via Western blot. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05 vs. Ctrl or siNC group.