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Research Paper Volume 13, Issue 9 pp 12537—12551

Silencing long noncoding RNA NEAT1 alleviates acute liver failure via the EZH2-mediated microRNA-139/PUMA axis

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Figure 5a. miR-139 suppressed production of pro-inflammatory cytokines by targeting PUMA. (A) Prediction of binding of miR-139 to PUMA-3’UTR by TargetScan database (http://www.targetscan.org/vert_71/). (B) RT-qPCR to determine the expression levels of PUMA in serum samples of patients with ALF, ALF murine liver tissues, and LPS-induced PBMCs. (C) Results of Western blot analysis to determine the protein levels of PUMA in ALF murine liver tissues, and LPS-induced PBMCs. (D) Dual-luciferase reporter gene assays to analyze the target relationship between miR-139 and PUMA.