Research Paper Volume 13, Issue 9 pp 12780—12799

Figure 4. Overexpression of circRNA_30032 enhances TGF-β1-induced expression of collagen I, collagen III, and fibronectin in BUMPT cells. (A) RT-qPCR analysis shows circRNA_30032 levels in control and circRNA_30032-overexpressing BUMPT cells treated with or without TGF-β1for 24 h. (B) Representative western blot images and (C–E) densitometric measurements show the levels of (B, C) collagen I, (B, D) collagen III, and (B, E) fibronectin proteins in control and circRNA_30032-overxpressing BUMPT cells treated with or without TGF-β1for 24 h. Note: The data are expressed as means ± SD (n = 6). # denotes p < 0.05 when comparing control vector plus TGF-β1 group or circRNA_30032 overexpression vector plus saline group vs. control vector plus saline group; * denotes p < 0.05 when comparing circRNA_30032 overexpression vector plus TGF-β1 group vs. control vector plus TGF-β1 group.