Research Paper Volume 13, Issue 9 pp 12780—12799

Figure 8. CircRNA_30032 promotes expression of pro-fibrosis factors in TGF-β1 treated BUMPT cells by suppressing miR-96-5p. (A, B) RT-qPCR analysis shows (A) circRNA_30032 and (B) RT-qPCR analysis of miR-96-5p expression. (C) Representative western blot images and (D, H) densitometric measurements show levels of (C, D) collagen I, (C, E) collagen III, (C, F) fibronectin, (C, G) HBEGF, and (C, H) KRAS proteins in BUMPT cells co-transfected with 100 nM circRNA_30032 siRNA plus anti-miR-96-5p or anti-miRNA, and then treated with TGF-β1 for 24h. Note: The data are expressed as means ± SD (n = 6). # denotes p < 0.05 when comparing anti-miRNA plus TGF-β1 group vs. anti-miRNA plus saline group; * denotes p < 0.05 when comparing circRNA_30032 siRNA plus TGF-β1 group vs. anti-miRNA plus TGF-β1 group; ^ denotes p < 0.05 when comparing circRNA_30032 siRNA plus anti-miR-96-5p plus TGF-β1 group vs. circRNA_30032 siRNA plus TGF-β1 group.