Research Paper Volume 13, Issue 9 pp 12896—12918

Figure 9. P4HA1 promoted the metastasis of OS in vitro. (A) Western blotting analysis of the expression of P4HA1 in human osteoblastic cell line and osteosarcoma cell lines. (B) Western blotting analysis of the expression of P4HA1 in HOS and 143B cell lines after transfection of si-control, si-P4HA1-1 and si-P4HA1-2. (C) Wound healing assay analysis in HOS and 143B cell lines after transfection of si-control and si-P4HA1-1. Representative images of migration were shown in the right panel. The degrees to which the wounds healed was shown in the histogram. (D) Transwell invasion assay analysis in HOS and 143B cell lines after transfection of si-control and si-P4HA1-1. Representative images of invasion were shown in the right panel. The proportions of invading cells were shown in the histogram. The bars indicate the mean±s.d. Statistically significant differences (t-test), **P<0.01, ***P<0.001. (E) The number of lung metastasis nodules formed by P4HA1-knockdown 143B cells and control cells in orthotopic osteosarcoma implanted mice was shown in the right panel. Statistically significant differences (t-test), **P<0.01. Representative images of lung morphology and lung metastatic nodules formed by P4HA1-knockdown 143B cells and control cells were shown in the left panel. Corresponding HE staining was shown in the middle panel. Scale bars = 200 μm.