Research Paper Volume 13, Issue 10 pp 13788—13806

Figure 3. Redd1 suppression attenuates Dox-induced senescence in cardiomyocytes. (A) Representative images of SA-β-gal staining in Dox-challenged H9c2 cardiomyocytes following Redd1 knockdown or overexpression. (B) Quantitative analysis of SA-β-gal activity; the number of SA-β-gal positive cells observed in at least 6 separate microscopic fields is indicated (n = 6 per group). ^*p < 0.05 vs. the Lv-NC group. ^&p < 0.05 vs. the Lv-shNC group. (C–F) Effects of Redd1 overexpression and knockdown on p16^INK4a and p21 expression in Dox-challenged H9c2 cardiomyocytes (n = 3 per group). ^*p < 0.05 vs. the Lv-NC group. ^&p < 0.05 vs. the Lv-shNC group. (G–J) Effect of Redd1 overexpression and knockdown on IL-1β, IL-6, IL-12, and TNFα mRNA in Dox-stimulated H9c2 cardiomyocytes (n = 3 per group). Data are mean ± SEM. ^*p < 0.05 vs. the Lv-NC group. ^&p < 0.05 vs. the Lv-shNC group.