Research Paper Volume 13, Issue 10 pp 14015—14038

Figure 1. NUPR1 upregulated is predominantly found in ccRCC and associated with poor prognosis. (A) Comparison of NUPR1 mRNA expression between ccRCC and normal kidney tissue from TCGA-KIRC dataset. (B) Relative mRNA level of NUPR1 in ccRCC cell lines compared with human renal proximal tubular epithelial cell line HK-2. (C) Western blot showed NUPR1 protein expression in ccRCC cell lines and HK-2. (D) Relative mRNA level of NUPR1 in twelve ccRCC tissues and paired normal kidney tissues. (E) Immunostaining of NUPR1 expression in 117 ccRCC tissues. Immunostaining intensity was scored as 0 negative, 1 weak positive, 2 moderate positive and 3 strong positive. (F) Overexpression of NUPR1 was associated with higher pathologic T stage, metastasis, elevated clinical stage and histologic grade in TCGA-KIRC dataset. (G) Kaplan-Meier curves of overall survival and disease-free survival time between low (n=135) and high (n=389) NUPR1 mRNA level groups in TCGA-KIRC dataset. The cutoff value was the mean of NUPR1 mRNA level in 524 ccRCC tissues. (H) Kaplan-Meier curves of overall survival and disease-free survival time between low (n=59) and high (n=58) NUPR1 immunostaining intensity groups in cohorts from Shandong Provincial Hospital. The median IHC score was used as the cutoff value. (*p < 0.05, **p < 0.01, ***p < 0.001). (N.S., No statistical significance). ccRCC: clear cell renal cell carcinoma; KIRC: Kidney renal clear cell carcinoma; TCGA: The Cancer Genome Atlas database.