Research Paper Volume 13, Issue 9 pp 13287—13299

Figure 3. CircRNA 0001445 is taken up and upregulated in glioma cells treated with glioma-derived exosomes. (A) Heat map of the expression profile for differentially expressed circRNAs between glioma cells treated with or without exosomes isolated from A172 or U373 cells, as detected by RNA sequencing. (B) The expression of circRNA 0001445 in glioma cells treated with exosomes isolated from A172 or U373 cells. (C) RFP-labeled exosomal circRNA 0001445 was taken up by GFP-labeled recipient A172 or U373 cells, as illustrated by FISH. Scale bar = 25 μm. (D) The expression of circRNA 0001445 in glioma cells treated with circPACRGL siRNA (hsa_circ_0001445-si) or siRNA negative control (NC-si). (E) The expression of circRNA 0001445 in glioma cells treated siRNA negative control (NC-si), circRNA 0001445 siRNA (hsa_circ_0001445-si) or hsa_circ_0001445-si plus exosomes isolated from A172 or U373 cells. Data were represented as mean ± SD. *** p < 0.001.