Figure 8. TGFβ signaling was essential for HNF1A-AS1 mediated cell migration and invasion. (A) Transwell was performed to analyze the effect of SB431542 on TGFβ signaling pathway. (B) TGFβ signaling transduction by si-HNF1A-AS1 and/or SB431542 was validated by western blot. (C) Western blot was used to analyze the effect of SB431542 on EMT markers after knockdown of HNF1A-AS1. *p < 0.05, **p < 0.01.