Research Paper Volume 13, Issue 9 pp 12334—12358

Figure 4. Sirt6 overexpression protects cardiomyocytes from senescence. (A) Flow cytometric analysis of senescence induced H9c2 cardiomyocytes. The Gated region indicates percentages of cells expressing low levels of SIRT6. Values are the average of three independent experiments, Mean ± SE. (B) Quantification of SIRT6 in samples shown in (A). (C) Representative images of β-galactosidase staining of control and senescence induced cardiomyocytes transduced with empty or SIRT6 adenovirus scale bars, 100 μm. (D) Quantification of senescent cells in (C). Values are average of three independent experiments, Mean ± SE. (E) Flow cytometric analysis of control and senescence induced cardiomyocytes transduced with empty or SIRT6 adenovirus. The Gated region indicates the percentages of cells expressing p16. Values are the average of three independent experiments, Mean ± SE. (F) Quantification of p16 levels in samples shown in (E).