Research Paper Volume 13, Issue 10 pp 14159—14169

Figure 2. Overexpression of AK136714 inhibits atherosclerosis. (A) qPCR was used to detect the expression of AK136714 in vascular tissue of each group. (B) Vascular oil red O staining was performed to detect the degree of atherosclerosis. (C) ELISA was used to assess inflammatory factor expression. (D) Oil red O staining was performed to detect the degree of vascular injury. (E) Immunofluorescence detection showed that AK136714 is mainly in the cytoplasm of endothelial cells and colocalizes with the endothelial marker CD31. (F) Immunofluorescence staining of CD31 showed that high fat damages endothelial cells, and AK136714 knockdown can reverse endothelial cell damage. (n = 8, *P < 0.05, **P < 0.01).