Figure 6. AK136714 inhibits endothelial cell apoptosis by binding to FOXO3. (A) Flow cytometry was used to analyze the apoptosis of endothelial cells. (B) TUNEL staining was used to analyze the apoptosis of endothelial cells. (C) Western blot was used to detect Bim expression in each group. (D) qPCR was performed to evaluate the expression of bax. (E) Western blot for detection of apoptotic protein expression in each group (n=6, *P < 0.05, **P < 0.01).