Research Paper Volume 13, Issue 10 pp 14198—14218

Figure 4. MiR-328-5p functions as a tumor suppressor in ESCC cells. (A) QRT-PCR analysis shows the expression levels of miR-328-5p in Kyse30 and TE-1 cells transfected with negative control (miR-NC), miR-328-5p mimics, and miR-328-5p inhibitors. (B) Colony formation assay results show the viability of ESCC cells transfected with miR-328-5p mimics and miR-328-5p inhibitors. (C) Flow cytometry analysis shows the apoptotic rate of ESCC cells transfected with miR-328-5p mimics and miR-328-5p inhibitors. (D) Flow cytometry analysis shows cell cycle distribution of ESCC cells transfected with miR-328-5p mimics and miR-328-5p inhibitors. (E) Western blot analysis demonstrates the expression levels of pro-apoptotic (cleaved caspase 3 and Bax) and cell cycle-related proteins (CDK4 and cyclinD1) in ESCC cells transfected with miR-328-5p mimics and miR-328-5p inhibitors. (F–G) wound healing and Transwell invasion assays show the (F) migration and (G) invasiveness of ESCC cells transfected with scrambled, si-LOC146880#2, miR-328-5p inhibitors, miR-328-5p inhibitors plus si-LOC146880#. (H) Western blot analysis shows the expression levels of EMT-related proteins, namely, E-cadherin, N-cadherin, and vimentin in ESCC cells transfected with scrambled, si-LOC146880#2, miR-328-5p inhibitors, miR-328-5p inhibitors plus si-LOC146880#.