Research Paper Volume 13, Issue 10 pp 14416—14432

Figure 3. HDAC5 results in promotion of epilepsy via HIF1α and PFKFB3 upregulation in epilepsy hippocampal neurons. (A) HIF1α and PFKFB3 protein expression in mice (n = 10). (B) HIF1α and PFKFB3 protein expression in cells. (C) Protein expression of HDAC5, HIF1α, and PFKFB3 in cells. (D) mRNA expression of HDAC5, HIF1α, and PFKFB3 in cells. (E) HIF1α protein expression in the nucleus and cytoplasm. (F) IP detected the binding of HDAC5 to HIF1α. (G) CHIP detected the enrichment of HIF1α in PFKFB3 promoter. (H) Cell apoptosis determined by flow cytometry. (I) SOD levels. (J) GSH-Px levels. (K) MDA levels. (L) DNA 8-OHdG levels. (M) IL-1β, TNF-α, and IL-6 levels. * p < 0.05 vs. control cells, blank control cells, epilepsy cells treated with sh-NC, or epilepsy cells treated with sh-NC + oe-NC; # p < 0.05 vs. epilepsy cells treated with sh-HDAC5 + oe-NC. Data were expressed as mean ± standard deviation. Data between two groups were compared independent sample t-test. Data among multiple groups were compared by one-way analysis of variance and post-hoc Tukey’s test. Experiments were repeated three times.