Chronic alcohol exposure promotes HCC stemness and metastasis through <i>β</i>-catenin/miR-22-3p/TET2 axis

Danlei Chen; Yan Yan; Xinyi Wang; Suzhi Li; Yan Liu; Dandan Yu; Yongjing He; Ruiqing Deng; Yakun Liu; Mei Xu; Jia Luo; Hongjun Gao; Siying Wang

doi:doi:10.18632/aging.203059

← Back

Research Paper Volume 13, Issue 10 pp 14433—14455

Chronic alcohol exposure promotes HCC stemness and metastasis through β-catenin/miR-22-3p/TET2 axis

Figure 7. Effects of salinomycin/ β-catenin siRNA and alcohol exposure on β-catenin signaling and miR-22-3p. (A) The effect of salinomycin and alcohol exposure on β-catenin signaling in HepG2 or SMMC-7721 cells. (B) The protein levels of p-GSK-3β^ser9 and active β-catenin in HepG2 cells were qualified and shown in column graph. *P< 0.05. (C) The protein levels of p-GSK-3β^ser9 and active β-catenin in SMMC-7721 cells were qualified and shown in column graph. *P< 0.05. (D) The effect of salinomycin and alcohol exposure on miR-22-3p in HepG2 cells. *P< 0.05. (E) The effect of salinomycin and alcohol exposure on miR-22-3p in SMMC-7721 cells. *P< 0.05. (F) The effect of β-catenin siRNA and alcohol exposure on β-catenin signaling in HepG2 or SMMC-7721 cells. (G) The protein levels of p-GSK-3β^ser9 and active β-catenin in HepG2 cells were qualified and shown in column graph. *P< 0.05. (H) The protein levels of p-GSK-3β^ser9 and active β-catenin in SMMC-7721 cells were qualified and shown in column graph. *P< 0.05. (I) The effect of β-catenin siRNA and alcohol exposure on miR-22-3p in HepG2 cells. *P< 0.05. (J) The effect of β-catenin siRNA and alcohol exposure on miR-22-3p in SMMC-7721 cells. *P< 0.05.