Research Paper Volume 13, Issue 10 pp 13474—13495
Ganoderma lucidum stimulates autophagy-dependent longevity pathways in Caenorhabditis elegans and human cells
Figure 6. G. lucidum-derived sub-fraction 10K-2 represses the mTOR pathway in human cells. (A) Inhibition of the mTOR pathway by G. lucidum (GL) sub-fraction 10K-2. Huh7 liver cells were treated with control medium (i.e., Dulbecco’s modified Eagle’s medium, DMEM), 10K-1 or 10K-2 for 4 hrs, prior to Western blot analysis. (B) Protein intensity was evaluated by densitometry and normalized against actin. (C) Sub-fraction 10K-2 inhibits the mTOR pathway in IMR-90 cells. Cells cultured in Eagle’s miminum essential medium (EMEM) were processed as above for Western blot analysis. (D) Densitometry analysis of the results shown in (C). *p<0.05; **p<0.01; ***p<0.001.