Research Paper Volume 13, Issue 12 pp 16471—16484

Figure 2. HCG11 depletion suppressed proliferation, cycle, colony formation, mobility of PANC-1 and AsPC-1 cells but promoted their apoptosis. (A, B) CCK-8 assay was used to examine the viability of PANC-1 and AsPC-1 cells after transfected with si-HCG11. (C, D) After transfection with si-HCG11, flow cytometry assay was performed to detect the cell cycle of PANC-1 and AsPC-1 cells. (E) Plate clone formation assay was conducted to measure the number of cell clones in PANC-1 and AsPC-1 cells. (F) After knockdown of HCG11, flow cytometry assay was applied to detect the apoptotic rates in PANC-1 and AsPC-1 cells. (G) Transwell assay was used to determine the invaded number of PANC-1 and AsPC-1 cells after knockdown of HCG11. ^*p<0.05, ^**p<0.01, ^***p<0.0001 vs. siRNA NC.