Research Paper Volume 13, Issue 12 pp 16471—16484

Figure 4. miR-579-3p directly targeted MDM2 to suppress MDM2 expression. (A) qPCR assay was used to examine MDM2 expression in 20 pairs of pancreatic carcinoma tissues and corresponding normal tissues. ^***p<0.001 vs. normal tissues. (B) MDM2 expression in pancreatic carcinoma cell lines (BxPC-3, Capan-2, SW1990, PANC-1 and AsPC-1) was also detected by qPCR assay. ^**p<0.01, ^***p<0.001 vs. HPDE6-C7. (C, D) Analysis from qPCR revealed that MDM2 expression was positively related to HCG11 expression, and negatively associated with miR-579-3p expression. (E) The binding sites between miR-579-3p and MDM2 were exhibited. (F, G) The luciferase activity in PANC-1 and AsPC-1 cells was measured by the luciferase reporter assay after transfected with miR-579-3p mimic/NC and MDM2-mut/wt vector. (H, I) MDM2 mRNA and protein expression levels in pancreatic carcinoma cells were detected by qPCR and western blotting assays when treated with miR-579-3p mimic or NC. ^*p<0.05, ^**p<0.01 vs. mimic NC. (J–M) MDM2 mRNA and protein expression levels in pancreatic carcinoma cells were detected by qPCR and western blotting assays when treated with siRNA NC, si-HCG11, pcDNA3.1, and pcDNA3.1-HCG11. ^*p<0.05, ^**p<0.01 vs. siRNA NC, ^##p<0.01, ^###p<0.001 vs. pcDNA3.1.