Research Paper Volume 13, Issue 16 pp 20192—20217

Figure 3. Hsa-circ-0007292 promotes the osteogenic differentiation of PLL cells. (A) qRT-PCR assays detected the transfection efficiency of siRNAs targeting hsa_circ_0007292 in OPLL cells (n=3). (B) The osteogenic properties of OPLL cells transfected with siRNA of hsa_circ_0007292 and negative control were analyzed using alkaline phosphatase staining after osteo-induction for 10 days (scale bars, 100 um). (C, D) Knockdown of hsa_circ_0007292 inhibited the mRNA and protein expression of osteogenesis-related markers in OPLL cells (n=3). (E) Hsa_circ_0007292 was significantly upregulated after transfection of non-OPLLs with the overexpression vector. (F) The osteogenic properties of non-OPLL cells transfected with overexpression plasmid and blank vector were analyzed using alkaline phosphatase staining after osteo-induction for 10 days (scale bars, 100 um). (G, H) Overexpression of hsa_circ_0007292 promoted osteogenesis-related markers expression in non-OPLL cells at both mRNA and protein levels (n=3). All tests were conducted at least three times. Data are exhibited as mean ± SD. ns (not significant, P>0.05), *P < 0.05, **P < 0.01.