Research Paper Volume 13, Issue 16 pp 20192—20217

Figure 6. Hsa_circ_0007292 promotes the osteogenic differentiation of PLL cells by sponging miR-508-3p. (A) The suppressed ALP staining of OPLL cells caused by si-circ-0007292 was rescued by miR-508-3p-inhibitor. (B, C) The hsa_circ_0007292 knockdown-induced decrease of osteogenic differentiation-related markers was partially restored by miR-508-3p inhibitor at both the mRNA and protein levels(n=3). (D) The enhanced ALP staining of non-OPLL cells treated with hsa_circ_0007292 overexpression plasmid was reversed by miR-508-3p mimic. (E, F) The miR-508-3p mimic rescued the osteogenic effects caused by hsa_circ_0007292 overexpression, as explored by qRT-PCR and Western Blot(n=3). All experiments were conducted at least three times. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P<0.001.