Research Paper Volume 13, Issue 16 pp 20383—20394

Figure 3. MiR-224-5p is targeted and negatively regulated by hsa_circ_0134111. (A) Diagram showing binding sites for miR-224-5p in the hsa_circ_0134111 sequence and nucleotide sequence of the mutant hsa_circ_0134111 construct. (B) Chondrocytes were treated with miR-224-5p NC, miR-224-5p agomir, or miR-224-5p antagomir and RT-qPCR was performed to measure miR-224-5p levels in cells. (C) A dual-luciferase reporter assay was used to evaluate binding of miR-224-5p to hsa_circ_0134111. (D) FISH experiment was performed to detect the co-localization of hsa_circ_0134111 and miR-224-5p in human primary chondrocytes. (E) CCK-8 assay was used to detect cell viability. (F, G) Flow cytometric analysis of apoptosis. (H) Chondrocytes were treated with miR-515-5p NC or miR-515-5p antagomir and RT-qPCR was performed to measure miR-515-5p levels in cells. (I) CCK-8 assay was performed to detect cell viability. ^**P<0.01, compared with control; ^##P<0.01, compared with IL-1β. ^{^^}P<0.01, compared with IL-1β + hsa_circ_0134111 siRNA1.