Research Paper Volume 13, Issue 16 pp 20050—20080

Figure 11. Changes in mitochondrial respiration and glycolysis level of MRC5-SV40 cells after Abisil treatment. (A) After 16 hours of Abisil treatment, mitochondrial respiration reflected by the level of oxygen consumption rate (OCR) in control (DMSO 0.5%) or Abisil (50 μg/ml) groups (n = 4 per group), following the injection of oligomycin, FCCP and antimycin A/rotenone. (B) the extracellular acidification rate (ECAR) of the medium following injection of glycose, oligomycin and 2 desoxyglucose. (C) The rates of glycolysis level, glycolytic capacity and glycolytic reserve (n = 4 per group). Bars represent mean values with SD, **p < 0.01, ***p < 0.001 (t-test). Glycolytic level – the basal rate of conversion of glucose to pyruvate/lactate; glycolytic capacity – the maximal rate of glycolysis that can be rapidly achieved by a cell; glycolytic reserve – the difference between the glycolytic capacity and the basal glycolytic rate.