Research Paper Volume 13, Issue 16 pp 20808—20819
LINC01087 inhibits glioma cell proliferation and migration, and increases cell apoptosis via miR-384/Bcl-2 axis
Figure 2. Down-regulating LINC01087 can inhibit the proliferation of glioma cells and induce apoptosis. (A) The relative expression of LINC01087 in the constructed sh-LINC01087 plasmid was tested by qRT-PCR. (B) The relative expression in glioma cells transfected with sh-LINC01087#1 was detected by qRT-PCR. (C) Viability of glioma cells transfected with sh-LINC01087#1 was measured by CCK-8 test. (D) Colony-formation ability changes of glioma cells transfected with sh-LINC01087#1 were tested by clone experiment. (E) Proliferation of glioma cells transfected with sh-LINC01087#1 was analyzed by Edu assay. (F) The apoptosis rate of glioma cells transfected with sh-LINC01087#1 was detected by flow cytometry. (G) The changes of apoptosis-related proteins in glioma cells transfected with sh-LINC01087#1 were analyzed by WB. (H) The relative caspase-3 activity was measured by the caspase-3 activity kit. (*P < 0.05).