Research Paper Volume 13, Issue 18 pp 21975—21990

Figure 4. (A) Death receptor up-regulation is ERK1/2, p38 MAPK- and JNK-dependent. HCT116 cells (1 × 10⁶ cells/well) were treated with different dose of TS for 24 h or treated with 0.5 μg/ml TS for different time points. Whole-cell extracts were prepared and subjected to Western blotting for phosphorylated p38 MAPK, JNK, ERK1/2, c-jun, and Akt expression. The same blots were stripped and reprobed with p38, JNK, ERK1/2, c-jun, and Akt to verify equal loading. (B) TS mediates Sp1 and CHOP upregulation in human HCT116 cells in dose (i)- and time (ii)-dependent manner. HCT116 cells (1 × 10⁶ cells/well) were treated with different dose of TS (0-250 pg/ml) for 24 h or treated with 0.5 μg/ml TS for different time points. Whole-cell extracts were prepared and subjected to Western blotting for Sp1 and CHOP up-modulation and relative protein expression levels were quantified. The same blots were stripped and reprobed with β-actin to verify equal loading. (C) Thyme volatile oil inhibits STAT3 phosphorylation and JAK 2 levels in HCT-116 cell lines in a dose-dependent manner.