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Research Paper Volume 13, Issue 18 pp 22459—22473
miR-19b-3p relieves intervertebral disc degeneration through modulating PTEN/PI3K/Akt/mTOR signaling pathway
Figure 3. Overexpressing miR-19b-3p attenuated IL-1β/TNF-α/hydrogen peroxide-mediated HNPC apoptosis. In the in-vitro IVDD model, HNPCs were transfected with miR-19b-3p mimics. (A–C) The miR-19b-3p expression was determined by qRT-PCR. (D–F) The levels of MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS-5 were compared by WB. (G–I) CCK-8 experiment was implemented to test cell proliferation. (J–L) Cell apoptosis was monitored by TUNEL staining ** P <0.01, *** P <0.001 (vs. control group). & P <0.05, && P <0.01, &&& P <0.001 (vs. the IL-1β/TNF-α/hydrogen peroxide group). N=3.