Research Paper Volume 13, Issue 21 pp 24339—24348
Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway
Figure 4. Inhibiting Smad7 blocked the inhibitory effect of AMO-15a in SHG139 cells. (A) The siRNA of Smad7 (si-Smad7) or its negative control (si-NC) was transfected into SHG139 cells, and the reduction efficiency was examined. SHG139 cells were co-transfected with si-Smad7 and AMO-15a. (B) Wound healing assay was used to detect cell migration. Scale bar, 200 μm. (C) Transwell assay was performed to identify the invasion of SHG139 cells. Scale bar, 50 μm. (D) Western blot was used to tested the Smad7 protein expression and EMT relative markers (Vimentin, N-cadherin and E-cadherin). Data were expressed as mean ± SD.*P < 0.05.