Research Paper Volume 13, Issue 21 pp 24402—24416

Figure 3. TIMP1 is a direct target of miR-6745. (A) The four-way Venn diagram reveals the numbers of overlapping miRNAs obtained using four publicly available bioinformatics algorithms and the microarray-based TIMP1 signature. (B) Real-time RT-PCR was used to detect the relative expression of miR-6745 in normal gastric cells and gastric cancer cells. (C) Analysis of miR-6745 expression in 42 gastric carcinoma tissues and 42 adjacent normal gastric tissues. (D) Correlation between miR-6745 levels and TIMP1 levels in 42 gastric carcinoma tissues. (E) Nucleotide predicted miR-6745-binding site in the TIMP1 mRNA 3′-UTR. (F) Luciferase activities were measured in SGC-7901 and MGC-803 cells transfected with reporter plasmids containing WT-pmir-TIMP1 or MUT-pmir-TIMP1 together with miR-6745 mimics or miR-6745 mimic NC. Data represent the means ± SEM. **P < 0.01.