Research Paper Volume 13, Issue 23 pp 25138—25152

Figure 5. MYB is a miR-139-5p target gene. (A) Putative MYB and miR-139-5p sequence complementarity as predicted using StarBase. (B) Binding interactions between miR-139-5p and MYB were assessed via luciferase reporter assays in 293T cells cotransfected with MYB WT or MYB MUT constructs and miRNA NC or miR-139-5p mimics. *P<0.01 vs. control. (C) MYB expression was assessed via qPCR in GES-1, BGC-823, AGS, MGC-803, and HGC-27 cells. *P<0.01, **P<0.05 vs. GES-1 cells. (D) MYB expression levels in gastric cancer tissues in the TCGA database, *P<0.01. (E) MYB expression levels were assessed via qPCR in 26 pairs of gastric cancer tumors and paracancerous tissues, *P<0.01. (F) MYB mRNA levels were assessed in AGS and MGC-803 cells following miR-139-5p mimic transfection. *P<0.01 vs. the miR-NC group. (G) MYB protein levels were assessed in AGS and MGC-803 cells following miR-139-5p mimic transfection. (H) MYB mRNA levels in AGS and MGC-803 cells were assessed following miR-139-5p inhibitor transfection. *P<0.01 vs. the miR-NC group. (I) MYB protein levels were assessed in AGS and MGC-803 cells following miR-139-5p inhibitor transfection. (J) Pearson correlation analyses were used to evaluate the relationship between miR-139-5p and MYB expression in the TCGA database, R=-0.22, P<0.001. (K) Overall gastric cancer patient survival in the TCGA database was compared as a function of MYB expression (low vs. high; log-rank, P=0.01).