Research Paper Volume 13, Issue 23 pp 25241—25255
Endothelial cell–derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Figure 4. circHIPK3 sponges miR-106a-5p. (A) Putative miR-106a-5p binding sequences in wild-type or mutated circHIPK3. (B) Luciferase report assay showed the direct relationship between wild-type circHIPK3 and miR-106a-5p (**p < 0.01 mimics NC vs. miR-106-5p, **p < 0.01 inhibitor NC vs. miR-106-5p inhibitor). (C) Luciferase report assay showed the direct relationship between circHIPK3 mutation and miR-106a-5p. (D) qRT-PCR was performed to detect the amount of miR-106a-5p mRNA in VSMCs after the overexpression or knockdown of circHIPK3 (*p < 0.05 pcDNA3.1/circHIPK3 vs. pcDNA3.1, *p < 0.05 siR-circHIPK3 vs. siR-NC). (E) miR-106a-5p level was detected by qRT-PCR when cells were incubated with exosomes (**p < 0.01 NC-Exo vs. circHIPK3-Exo).