Research Paper Volume 13, Issue 23 pp 25241—25255
Endothelial cell–derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway
Figure 7. Foxo1 serves as a transcription factor to regulate Vcam1 expression. (A) qRT-PCR, (B) western blot analysis, and (C) IF staining detected Vcam1 mRNA and protein levels in VSMCs knocking down Foxo1 (*p < 0.05 siR-Foxo1 vs. siNC). (D) qRT-PCR and (E) western blot analysis detected Vcam1 mRNA and protein levels in VSMCs (*p < 0.05 ECs-Exo-HG vs. ECs-Exo-NG, **p < 0.01 miR-106a-5p vs. NC). (F) CCK-8 was used to detect cell viability in VSMCs knocking down Vcam1 (*p < 0.05 siR-Vcam1 vs. siNC). (G–H) The expression of Foxo1 (G) and Vcam1 (H) in the aorta of db/db mice and db/m mice by IF.