Research Paper Volume 13, Issue 23 pp 25377—25392

Figure 1. Effects of Tartary buckwheat flavonoids (TBF) on mammary gland tissues of mice induced by high fat diet (HFD) during pregnancy and lactation. 1 g/L TBF was dissolved in 0.1% carboxymethylcellulose sodium solution and was ingested by mice by drinking water. Drinking water administration began on the first day of mating and stopped one week after delivery, and then mammary tissue was collected. The mice mammary tissue was made into paraffin sections, which were used for H&E staining and Masson staining. (A–D) Mice mammary tissue; (E–H) H&E staining result of mammary tissue; (I–L) Masson result of mice mammary, blue represents collagen fiber, red represents muscle fiber; (M) Mammary gland damage score; The arrow represents the lesion of mammary tissue; (N) Schematic diagram of the mice experiment cycle; The arrow represents the lesion of mammary tissue; H&E and Masson are the results of 200× magnification, Scale bar: 100 μm. The data error was based on SEM, three independent repeated experiments were performed; ^#p < 0.01 vs. No treatment group (NT) group; ^∗∗p < 0.01 vs. HFD group.